![]() ![]() The ROCK MAKER Crystallization Software can automatically generate experiments for each ISO step, adjusting the precipitant concentration of each condition based on user scores. After the first iteration, every subsequent round of ISO takes into account the scores of the last two experiments to calculate the precipitant concentration in the next experiment. This ISO will then be performed on the newly created experiment (round 2) and such iterations will be done a few more times in a series. For example, if the initial experiment yielded a clear score, the next ISO experiment will increase the precipitant concentration. Based on these scores, the ISO process creates a new experiment wherein the concentration of the precipitant from each condition is altered - either increased or decreased, to get into the nucleation zone in the protein solubility curve. After images are collected on this experiment, a user classifies each of the images by scoring them as either Clear, Crystal, Light Precipitate, or Heavy Precipitate. Using a premade screen, an initial screening experiment is set up. ISO is a highly automated optimization process that can help researchers yield protein crystals from conditions that initially do not look promising. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |